Взаимоотношения гипоталамо-гипофизарно-надпочечниковой и пептидергической систем гипоталамуса у животных с экспериментальным сахарным диабетом

Abstract

The status of the hypollialamo-pituitary adre nal system (HPAS) and the group of regulatory neuropeptides synthesized iu the zone of the medial small cell hypothalamic subnticleus, the principal site of production of corticotropin re leasing factor, were studied in Wistar rats. HPAS was studied by radioimmunological and morphohistochemical methods, the neu ropeptidergic system by indirect immunofluorescence which helps measure the levels of neurotensin, bombesin, vasoactive intestinal peptide, leu- and mel-enkephalines, calcitonin-gene-related pep lide, and cholecystokinin in the neurones and medial eminence of the hypothalamus. Development of diabetes mellitus iu rats was found to be associated with an increase of HPAS activity and alteration of the peptidergic system presenting as an increase of the number of identified immunoreactive neurones and changed con tent of neuropeptides in neurones and of their concentration in the medial eminence.