Development and Validation of UV Spectrometric Method for Estimation of Aminexil (Kopexil) in Bulk

Abstract

Background. Lately Aminexil (Kopexil) is widely added in the hair products to thicken the roots, but there is no reported simple, fast and accurate method to determine quantity of Aminexil just by UV-vis spectrometer. Objective: to develop method of Aminexil determination by UV spectroscopy and validate it for linearity, range, accuracy, precision, ruggedness and robustness. Methods. The conditions and concentration of Aminexil were optimized according to the linear correlation with maximum UV absorbencies. Results. A simple, precise, accurate, reproducible and low cost UV-spectrophotometric method has been developed and validated for the quantification of Aminexil in bulk. The linearity was found for UV absorption maximum at the 284 nm in the concentration ranges of 1.0–1.8 mg/100mL in 0.1 M HCl solution with r2=0.9987. The LOD was 0.22 mg/100mL, while LQD was 0.65 mg/100mL. Conclusion: The proposed ready-to-use technique can be successfully used for determination of Aminexil in bulk and for future studies in pharmaceutical formulations.